Practical procedures for ectopic induction of gene expression in zebrafish embryos using Bhc-diazo-caged mRNA.

Preparation and affinity-based purification of caged linear DNA for light-controlled gene expression in mammalian cells.

A facile and useful method for preparing caged DNAs was developed. The method includes a caging reaction of a linear dsDNA having a minimal sequence of protein expression with Bio-Bhc-diazo and affinity separation of the caged DNA. Effective suppression and photo-mediated restoration of protein expression were demonstrated.

O-12: Study of Expression of DevelopmentalGenes in SCNT Cloned Embryos

(SCNT) embryos of buffaloes. 2. To study gene expression profile of important developmental genes at different stages of SCNT cloned embryo. 3. To study epigenetic reprogramming during early developments of SCNT embryos Materials and Methods: Expression analysis of developmental genes was done in different (ovarian granulose and cumulus and skin fibroblasts) donor cells; in vitro maturing oocyt...

Use of an IRES bicistronic construct to trace expression of exogenously introduced mRNA in zebrafish embryos.

To understand gene function in developing vertebrate embryos, co-injection of an mRNA for a reporter protein and an mRNA for a testing factor is widely used. However, because of the mosaic segregation of injected nucleic acids during early embryogenesis, whether both mRNAs are translated in the same cell remains uncertain. In the present study, we tested a new system of tracing the expression o...

Hematopoietic regulatory domain of gata1 gene is positively regulated by GATA1 protein in zebrafish embryos.

Expression of gata1 is regulated through multiple cis-acting GATA motifs. To elucidate regulatory mechanisms of the gata1 gene, we have used zebrafish. To this end, we isolated and analyzed zebrafish gata1 genomic DNA, which resulted in the discovery of a novel intron that was unknown in previous analyses. This intron corresponds to the first intron of other vertebrate Gata1 genes. GFP reporter...

The Expression of The Autophagy Gene Beclin-1 mRNA and Protein in Ectopic and Eutopic Endometrium of Patients with Endometriosis